Early intermediates in bacteriophage lambda prohead assembly II. Identification of biologically active intermediates
Identifieur interne : 002340 ( Istex/Checkpoint ); précédent : 002339; suivant : 002341Early intermediates in bacteriophage lambda prohead assembly II. Identification of biologically active intermediates
Auteurs : Jarema Kochan [Canada] ; Helios Murialdo [Canada]Source :
- Virology [ 0042-6822 ] ; 1983.
English descriptors
- Teeft :
- Active intermediates, Active proheads, Aliquot, Assay, Bacteriophage, Bacteriophage lambda, Bacteriophage lambda prohead, Bacteriophage lambda prohead assembly, Becker, Beckman ultracentrifuge, Biological activity, Casjens, Cell extracts, Column elution conditions, Complementation, Complementation reaction, Extract, Ferrucci, Final volume, Fraction number, Gene products, Genes groel, Glycerol gradients, Gpgroel, Gpnu3, Groe, Groe functions, Groel, Hendrix, Hohn, Kochan, Lambda, Lambda prohead assembly, Liquid nitrogen, Marker rescue, Molecular nature, Monomeric, Morphogenesis, Murialdo, Mutation, Nacl, Phage, Phage lambda, Polymer, Proc, Prohead, Prohead assembly, Proheads, Radioactively, Room temperature, Sedimentation, Small oligomeric, Supernatant, Velocity sedimentation, Velocity sedimentation analysis, Water bath.
Abstract
Abstract: The morphogenesis of bacteriophage λ proheads is under the control of the four phage genes B, C, Nu3, and E, as well as the E. coli genes groEL and groES. It has been previously shown that extracts prepared from cells infected with a λ C−E− mutant accumulate biologically active gpB and gpNu3 (Murialdo, H., and Becker, A., J. Mol. Biol. 125, 57–74 (1978)). To characterize the nature of these intermediates in prohead assembly, extracts prepared from these cells were fractionated by DEAF-cellulose chromatography as well as velocity sedimentation. Intermediates containing gpB were identified by SDS-polyacrylamide gel electrophoresis and by their ability to be assembled into biologically active proheads in vitro. The results indicate that the most abundant, biologically active intermediate (greater than 98% of the gpB activity) is a 25 S gpB-containing polymer. A second biologically active intermediate (about 1% of the total gpB activity) was identified as a gpB-gpgroEL complex.
Url:
DOI: 10.1016/0042-6822(83)90537-8
Affiliations:
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<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Early intermediates in bacteriophage lambda prohead assembly II. Identification of biologically active intermediates</title><author><name sortKey="Kochan, Jarema" sort="Kochan, Jarema" uniqKey="Kochan J" first="Jarema" last="Kochan">Jarema Kochan</name></author><author><name sortKey="Murialdo, Helios" sort="Murialdo, Helios" uniqKey="Murialdo H" first="Helios" last="Murialdo">Helios Murialdo</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:D8BD8875BE16FEF63EAFBFCA7454F724EDA84EA4</idno><date when="1983" year="1983">1983</date><idno type="doi">10.1016/0042-6822(83)90537-8</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-B97ZHG15-D/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">002111</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">002111</idno><idno type="wicri:Area/Istex/Curation">002111</idno><idno type="wicri:Area/Istex/Checkpoint">002340</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">002340</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Early intermediates in bacteriophage lambda prohead assembly II. Identification of biologically active intermediates</title><author><name sortKey="Kochan, Jarema" sort="Kochan, Jarema" uniqKey="Kochan J" first="Jarema" last="Kochan">Jarema Kochan</name><affiliation wicri:level="4"><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Medical Genetics, University of Toronto, Medical Sciences Building, Toronto, Ontario M5S 1A8</wicri:regionArea><orgName type="university">Université de Toronto</orgName><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName></affiliation></author><author><name sortKey="Murialdo, Helios" sort="Murialdo, Helios" uniqKey="Murialdo H" first="Helios" last="Murialdo">Helios Murialdo</name><affiliation wicri:level="4"><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Medical Genetics, University of Toronto, Medical Sciences Building, Toronto, Ontario M5S 1A8</wicri:regionArea><orgName type="university">Université de Toronto</orgName><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="ISSN">0042-6822</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1983">1983</date><biblScope unit="volume">131</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="100">100</biblScope><biblScope unit="page" to="115">115</biblScope></imprint><idno type="ISSN">0042-6822</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0042-6822</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Active intermediates</term><term>Active proheads</term><term>Aliquot</term><term>Assay</term><term>Bacteriophage</term><term>Bacteriophage lambda</term><term>Bacteriophage lambda prohead</term><term>Bacteriophage lambda prohead assembly</term><term>Becker</term><term>Beckman ultracentrifuge</term><term>Biological activity</term><term>Casjens</term><term>Cell extracts</term><term>Column elution conditions</term><term>Complementation</term><term>Complementation reaction</term><term>Extract</term><term>Ferrucci</term><term>Final volume</term><term>Fraction number</term><term>Gene products</term><term>Genes groel</term><term>Glycerol gradients</term><term>Gpgroel</term><term>Gpnu3</term><term>Groe</term><term>Groe functions</term><term>Groel</term><term>Hendrix</term><term>Hohn</term><term>Kochan</term><term>Lambda</term><term>Lambda prohead assembly</term><term>Liquid nitrogen</term><term>Marker rescue</term><term>Molecular nature</term><term>Monomeric</term><term>Morphogenesis</term><term>Murialdo</term><term>Mutation</term><term>Nacl</term><term>Phage</term><term>Phage lambda</term><term>Polymer</term><term>Proc</term><term>Prohead</term><term>Prohead assembly</term><term>Proheads</term><term>Radioactively</term><term>Room temperature</term><term>Sedimentation</term><term>Small oligomeric</term><term>Supernatant</term><term>Velocity sedimentation</term><term>Velocity sedimentation analysis</term><term>Water bath</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The morphogenesis of bacteriophage λ proheads is under the control of the four phage genes B, C, Nu3, and E, as well as the E. coli genes groEL and groES. It has been previously shown that extracts prepared from cells infected with a λ C−E− mutant accumulate biologically active gpB and gpNu3 (Murialdo, H., and Becker, A., J. Mol. Biol. 125, 57–74 (1978)). To characterize the nature of these intermediates in prohead assembly, extracts prepared from these cells were fractionated by DEAF-cellulose chromatography as well as velocity sedimentation. Intermediates containing gpB were identified by SDS-polyacrylamide gel electrophoresis and by their ability to be assembled into biologically active proheads in vitro. The results indicate that the most abundant, biologically active intermediate (greater than 98% of the gpB activity) is a 25 S gpB-containing polymer. A second biologically active intermediate (about 1% of the total gpB activity) was identified as a gpB-gpgroEL complex.</div></front></TEI><affiliations><list><country><li>Canada</li></country><region><li>Ontario</li></region><settlement><li>Toronto</li></settlement><orgName><li>Université de Toronto</li></orgName></list><tree><country name="Canada"><region name="Ontario"><name sortKey="Kochan, Jarema" sort="Kochan, Jarema" uniqKey="Kochan J" first="Jarema" last="Kochan">Jarema Kochan</name></region><name sortKey="Murialdo, Helios" sort="Murialdo, Helios" uniqKey="Murialdo H" first="Helios" last="Murialdo">Helios Murialdo</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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